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PNKP Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96T60 |
| Other Accession | NP_009185.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57076 Da |
| Antigen Region | 109-138 aa |
| Gene ID | 11284 |
|---|---|
| Other Names | Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, PNKP |
| Target/Specificity | This PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PNKP. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PNKP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PNKP {ECO:0000303|PubMed:10446192, ECO:0000312|HGNC:HGNC:9154} |
|---|---|
| Function | Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193). |
| Cellular Location | Nucleus. Chromosome. Note=Localizes to site of double-strand breaks. |
| Tissue Location | Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
References
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Shen, J., et al. Nat. Genet. 42(3):245-249(2010)
Ali, A.A., et al. Nucleic Acids Res. 37(5):1701-1712(2009)
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