|Application ||WB, IHC-P, E|
|Calculated MW||80110 Da|
|Antigen Region||542-570 aa|
|Other Names||Heat shock protein 75 kDa, mitochondrial, HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1, TRAP1, HSP75|
|Target/Specificity||This TRAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-570 amino acids from the C-terminal region of human TRAP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRAP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.|
|Cellular Location||Mitochondrion. Mitochondrion inner membrane Mitochondrion matrix|
|Tissue Location||Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is higly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.|
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Provided below are standard protocols that you may find useful for product applications.
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).
Liu, D., et al. Cancer Lett. 296(2):194-205(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Nguyen, M.C., et al. Cancer Immunol. Immunother. 59(9):1313-1323(2010)
Landriscina, M., et al. Cancer Res. 70(16):6577-6586(2010)
Leav, I., et al. Am. J. Pathol. 176(1):393-401(2010)
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