RPH3A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9Y2J0 |
Other Accession | P47709, P47708, Q06846, NP_001137326.1, NP_055769.2 |
Reactivity | Human |
Predicted | Bovine, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 76872 Da |
Antigen Region | 658-686 aa |
Gene ID | 22895 |
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Other Names | Rabphilin-3A, Exophilin-1, RPH3A, KIAA0985 |
Target/Specificity | This RPH3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 658-686 amino acids from the C-terminal region of human RPH3A. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RPH3A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RPH3A |
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Synonyms | KIAA0985 |
Function | Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP- dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone (By similarity). |
Cellular Location | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P47709}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:P47709}. Postsynaptic cell membrane {ECO:0000250|UniProtKB:P47709}. Membrane {ECO:0000250|UniProtKB:P47709}; Peripheral membrane protein {ECO:0000250|UniProtKB:P47709} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).
References
Katkoori, V.R., et al. Front. Biosci. 13, 1050-1061 (2008) :
Smith, R., et al. J. Neurochem. 103(1):115-123(2007)
Lin, C.C., et al. J. Cell. Physiol. 211(2):316-326(2007)
Dalfo, E., et al. Neurobiol. Dis. 16(1):92-97(2004)
Rastaldi, M.P., et al. Am. J. Pathol. 163(3):889-899(2003)
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