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ZAP70 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP14145c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP14145c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ZAP70 Antibody (Center) - Product info | |
| Application | WB, FC, IHC
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| Primary Accession | P43403 |
| Other Accession | NP_001070.2, NP_997402.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 69872 Da |
ZAP70 Antibody (Center) - Additional info | |
| Gene ID 7535 | |
| Other Names ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase | |
| Target/Specificity This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human ZAP70. | |
| Dilution WB~~1:100~500WB~~1:1000 FC~~1:10~50 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ZAP70 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ZAP70 Antibody (Center) - Protein Information | |
| Name ZAP70 | |
| Synonyms SRK | |
| Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2 Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) | |
| Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) | |
| Tissue Location Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells | |
ZAP70 Antibody (Center) - Related products
AM2080a: ZAP70 Antibody (Ascites)
AP14145c: ZAP70 Antibody (Center)
AP14146a: ZAP70 Antibody (N-term)
AP3676a: Phospho-ZAP70-Y292 Antibody
AP7824c: ZAP70 Antibody (Center)
RI15782: ZAP70 predesign siRNA
DC07990: Human ZAP70 cDNA Clone
BP3658a: Phospho-ZAP70-Y319 Blocking Peptide
BP3659a: Phospho-ZAP70-Y492 Blocking Peptide
BP3660a: Phospho-ZAP70-Y493 Blocking Peptide
BP3661a: Phospho-ErbB2-pY1248(M) Blocking Peptide
BP3676a: Phospho-ZAP70-Y292 Antibody Blocking Peptide
BP7824c: ZAP70 Antibody (Center) Blocking Peptide
AJ1824b: ZAP-70 Antibody (C-term)
ZAP70 Antibody (Center) - Application data
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ZAP70 Antibody (Center) (Cat. #AP14145c) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).
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Western blot analysis of ZAP70 (arrow) using rabbit polyclonal ZAP70 Antibody (Center) (Cat. #AP14145c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZAP70 gene.
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ZAP70 Antibody (Center) (Cat. #AP14145c) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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ZAP70 Antibody (Center) (AP14145c)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ZAP70 Antibody (Center) - Research Areas
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BACKGROUND
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
REFERENCES
Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010) Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) : Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010) Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010) Trojani, A., et al. Cancer Biomark 6(1):1-9(2010)
