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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Map2k1 Antibody (C-term)   

Mouse Map2k1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Map2k1 Antibody (C-term) AP14153B
    Mouse Map2k1 Antibody (C-term) (Cat. #AP14153b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).
  • WB - Mouse Map2k1 Antibody (C-term) AP14153B
    Western blot analysis of Map2k1 (arrow) using rabbit polyclonal Mouse Map2k1 Antibody (C-term) (Cat. #AP14153b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.
  • WB - Mouse Map2k1 Antibody (C-term) AP14153B
    Mouse Map2k1 Antibody (C-term) (Cat. #AP14153b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).
  • IHC-P - Mouse Map2k1 Antibody (C-term) AP14153B
    Mouse Map2k1 Antibody (C-term) (AP14153b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31938
Other Accession Q01986, P29678, Q02750, Q63980, NP_032953.1
Reactivity Human, Mouse
Predicted Hamster, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43474 Da
Antigen Region 269-296 aa
Additional Information
Gene ID 26395
Other Names Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, Map2k1, Mek1, Prkmk1
Target/Specificity This Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-296 amino acids from the C-terminal region of mouse Map2k1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Map2k1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Map2k1
Synonyms Mek1, Prkmk1
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Research Areas
Citations (0)

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Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.


Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Yang, T., et al. J. Clin. Invest. 120(7):2474-2485(2010)
Cho, K.W., et al. J. Exp. Zool. B Mol. Dev. Evol. 312(8):901-911(2009)
Jo, C., et al. Cell. Signal. 21(12):1910-1917(2009)
Mandal, M., et al. Nat. Immunol. 10(10):1110-1117(2009)

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$ 295.00
$ 99.00
Cat# AP14153B
(40 western blots)
Availability: In Stock
Bulk Size
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