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HSF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HSF1 Antibody (N-term) AP14189a
    Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (N-term) (Cat. #AP14189a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
  • IHC-P - HSF1 Antibody (N-term) AP14189a
    HSF1 Antibody (N-term) (AP14189a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q00613
Other Accession P41154, P38532, Q08DJ8, NP_005517.1
Reactivity Human
Predicted Bovine, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57260 Da
Antigen Region 72-100 aa
Additional Information
Gene ID 3297
Other Names Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Target/Specificity This HSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the N-terminal region of human HSF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSF1
Synonyms HSTF1
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules
Research Areas
Citations (0)

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The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.


Hayashida, N., et al. EMBO J. 29(20):3459-3469(2010)
Sangle, G.V., et al. Endocrinology 151(9):4455-4466(2010)
Shunmei, E., et al. Mol. Cells 29(5):527-531(2010)
Kim, S.A., et al. Int. J. Oncol. 36(4):867-872(2010)
Wang, C., et al. J Transl Med 8, 44 (2010) :

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Cat# AP14189a
(40 western blots)
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