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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF20 Antibody (N-term)   

RNF20 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RNF20 Antibody (N-term) AP14192A
    RNF20 Antibody (N-term) (Cat. #AP14192a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF20 antibody detected the RNF20 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VTR2
Other Accession Q5DTM8, A2VDP1, NP_062538.5
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 113662 Da
Antigen Region 117-146 aa
Additional Information
Gene ID 56254
Other Names E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A
Target/Specificity This RNF20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the N-terminal region of human RNF20.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF20
Synonyms BRE1A
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Cellular Location Nucleus
Tissue Location Expressed in the normal brain and also in malignant gliomas (at protein level)
Research Areas
Citations (0)

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The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.


Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010)
Kim, J., et al. Cell 137(3):459-471(2009)
Liu, Z., et al. Mol. Biol. Cell 20(3):757-768(2009)
Shema, E., et al. Genes Dev. 22(19):2664-2676(2008)
Barber, T.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3443-3448(2008)

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$ 295.00
$ 99.00
Cat# AP14192A
(40 western blots)
Availability: In Stock
Bulk Size
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