|Application ||WB, E|
|Other Accession||Q5DTM8, A2VDP1, NP_062538.5|
|Calculated MW||113662 Da|
|Antigen Region||117-146 aa|
|Other Names||E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A|
|Target/Specificity||This RNF20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the N-terminal region of human RNF20.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.|
|Tissue Location||Expressed in the normal brain and also in malignant gliomas (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.
Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010)
Kim, J., et al. Cell 137(3):459-471(2009)
Liu, Z., et al. Mol. Biol. Cell 20(3):757-768(2009)
Shema, E., et al. Genes Dev. 22(19):2664-2676(2008)
Barber, T.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3443-3448(2008)
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