|Application ||WB, IHC-P, E|
|Other Accession||P02316, NP_004956.5|
|Calculated MW||10659 Da|
|Antigen Region||1-30 aa|
|Other Names||Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1, HMGN1, HMG14|
|Target/Specificity||This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HMGN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus|
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Provided below are standard protocols that you may find useful for product applications.
Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.
Rattner, B.P., et al. Mol. Cell 34(5):620-626(2009)
Cherukuri, S., et al. Mol. Biol. Cell 19(5):1816-1824(2008)
Zhu, N., et al. Mol. Cell. Biol. 27(24):8859-8873(2007)
Jiang, X.G., et al. Biochem. Biophys. Res. Commun. 345(4):1497-1503(2006)
Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :
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