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HMGN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HMGN1 Antibody (N-term) AP14219a
    HMGN1 Antibody (N-term) (Cat. #AP14219a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).
  • WB - HMGN1 Antibody (N-term) AP14219a
    Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) (Cat. #AP14219a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.
  • IHC-P - HMGN1 Antibody (N-term) AP14219a
    HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05114
Other Accession P02316, NP_004956.5
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 10659 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 3150
Other Names Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1, HMGN1, HMG14
Target/Specificity This HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGN1
Synonyms HMG14
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus
Research Areas
Citations (0)

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Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.


Rattner, B.P., et al. Mol. Cell 34(5):620-626(2009)
Cherukuri, S., et al. Mol. Biol. Cell 19(5):1816-1824(2008)
Zhu, N., et al. Mol. Cell. Biol. 27(24):8859-8873(2007)
Jiang, X.G., et al. Biochem. Biophys. Res. Commun. 345(4):1497-1503(2006)
Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :

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$ 295.00
$ 99.00
Cat# AP14219a
(40 western blots)
Availability: In Stock
Bulk Size
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