|Application ||WB, IHC-P, E|
|Calculated MW||72932 Da|
|Antigen Region||9-37 aa|
|Other Names||Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, ER protein 70, ERp70, Endoplasmic reticulum resident protein 72, ER protein 72, ERp-72, ERp72, PDIA4, ERP70, ERP72|
|Target/Specificity||This PDIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human PDIA4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDIA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The function of this protein remains unknown.
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Chen, W., et al. Biochem. J. 416(1):55-63(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Basrur, V., et al. J. Proteome Res. 2(1):69-79(2003)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.
1 2 3 4 5
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose