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PDIA4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PDIA4 Antibody (N-term) AP14224a
    PDIA4 Antibody (N-term) (Cat. #AP14224a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDIA4 antibody detected the PDIA4 protein (arrow).
    detail
  • IHC-P - PDIA4 Antibody (N-term) AP14224a
    PDIA4 Antibody (N-term) (AP14224a)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDIA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P13667
Other Accession NP_004902.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 72932 Da
Antigen Region 9-37 aa
Additional Information
Gene ID 9601
Other Names Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, ER protein 70, ERp70, Endoplasmic reticulum resident protein 72, ER protein 72, ERp-72, ERp72, PDIA4, ERP70, ERP72
Target/Specificity This PDIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human PDIA4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDIA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDIA4
Synonyms ERP70, ERP72
Cellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065)
Research Areas
Citations (0)
citation

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Background

The function of this protein remains unknown.

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Chen, W., et al. Biochem. J. 416(1):55-63(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Basrur, V., et al. J. Proteome Res. 2(1):69-79(2003)

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Anonymous
2016-08-23 21:15:32
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Species tested
Human
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose
$ 182.50
$ 70.00
Cat# AP14224a
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