PDIA4 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | P13667 |
Other Accession | NP_004902.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 72932 Da |
Antigen Region | 9-37 aa |
Gene ID | 9601 |
---|---|
Other Names | Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, ER protein 70, ERp70, Endoplasmic reticulum resident protein 72, ER protein 72, ERp-72, ERp72, PDIA4, ERP70, ERP72 |
Target/Specificity | This PDIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human PDIA4. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PDIA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PDIA4 |
---|---|
Synonyms | ERP70, ERP72 |
Cellular Location | Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The function of this protein remains unknown.
References
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Chen, W., et al. Biochem. J. 416(1):55-63(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Basrur, V., et al. J. Proteome Res. 2(1):69-79(2003)
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Anonymous
2016-08-23 21:15:32
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Species tested
Human
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |