|Application ||WB, IHC-P, E|
|Calculated MW||72932 Da|
|Antigen Region||9-37 aa|
|Other Names||Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, ER protein 70, ERp70, Endoplasmic reticulum resident protein 72, ER protein 72, ERp-72, ERp72, PDIA4, ERP70, ERP72|
|Target/Specificity||This PDIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human PDIA4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDIA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).|
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Provided below are standard protocols that you may find useful for product applications.
The function of this protein remains unknown.
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Chen, W., et al. Biochem. J. 416(1):55-63(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Basrur, V., et al. J. Proteome Res. 2(1):69-79(2003)
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose