|Application ||WB, E|
|Other Accession||NP_001112.2, NP_063968.1|
|Calculated MW||79155 Da|
|Antigen Region||552-580 aa|
|Other Names||Gamma-adducin, Adducin-like protein 70, ADD3, ADDL|
|Target/Specificity||This ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 552-580 amino acids from the C-terminal region of human ADD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ADD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping (PubMed:23836506). Binds to calmodulin.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Isoform 1 is ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Garcia-Barcelo, M.M., et al. Hum. Mol. Genet. 19(14):2917-2925(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Seidlerova, J., et al. Am. J. Hypertens. 22(1):21-26(2009)
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