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RNF19A Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9NV58 |
| Other Accession | Q2VJ60, P50636, NP_056250.3, NP_904355.1 |
| Reactivity | Human |
| Predicted | Mouse, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 90696 Da |
| Antigen Region | 384-413 aa |
| Gene ID | 25897 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF19A, 632-, Double ring-finger protein, Dorfin, RING finger protein 19A, p38, RNF19A, RNF19 |
| Target/Specificity | This RNF19A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-413 amino acids from the Central region of human RNF19A. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNF19A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF19A |
|---|---|
| Synonyms | RNF19 |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection. |
| Cellular Location | Membrane; Multi-pass membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients |
| Tissue Location | Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported.
References
Huh, J.W., et al. Gene 424 (1-2), 63-70 (2008) :
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Ishigaki, S., et al. Neurobiol. Dis. 25(2):331-341(2007)
Huang, Y., et al. J. Biol. Chem. 281(17):11610-11617(2006)
Ishigaki, S., et al. J. Biol. Chem. 279(49):51376-51385(2004)
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