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CIAO1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O76071 |
| Other Accession | Q32PJ6, NP_004795.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 37840 Da |
| Antigen Region | 155-183 aa |
| Gene ID | 9391 |
|---|---|
| Other Names | Probable cytosolic iron-sulfur protein assembly protein CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037}, WD repeat-containing protein 39 {ECO:0000255|HAMAP-Rule:MF_03037}, CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037}, WDR39 |
| Target/Specificity | This CIAO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the Central region of human CIAO1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CIAO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037} |
|---|---|
| Function | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:17937914, PubMed:23891004). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed:23891004). Seems to specifically modulate the transactivation activity of WT1 (PubMed:9556563). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633). |
| Cellular Location | Cytoplasm. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CIAO1 is an essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1.
References
Srinivasan, V., et al. Structure 15(10):1246-1257(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Higa, L.A., et al. Nat. Cell Biol. 8(11):1277-1283(2006)
Johnstone, R.W., et al. J. Biol. Chem. 273(18):10880-10887(1998)
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