|Application ||WB, E|
|Calculated MW||31288 Da|
|Antigen Region||190-219 aa|
|Other Names||Lck-interacting transmembrane adapter 1, Lck-interacting membrane protein, Lck-interacting molecule, LIME1, LIME|
|Target/Specificity||This LIME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the C-terminal region of human LIME1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LIME1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.|
|Cellular Location||Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell|
|Tissue Location||Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).
Ahn, E., et al. Blood 107(4):1521-1527(2006)
Tedoldi, S., et al. Blood 107(1):213-221(2006)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Brdickova, N., et al. J. Exp. Med. 198(10):1453-1462(2003)
Hur, E.M., et al. J. Exp. Med. 198(10):1463-1473(2003)
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