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LIME1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LIME1 Antibody (C-term) AP14247b
    LIME1 Antibody (C-term) (Cat. #AP14247b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LIME1 antibody detected the LIME1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H400
Other Accession NP_060276.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31288 Da
Antigen Region 190-219 aa
Additional Information
Gene ID 54923
Other Names Lck-interacting transmembrane adapter 1, Lck-interacting membrane protein, Lck-interacting molecule, LIME1, LIME
Target/Specificity This LIME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the C-terminal region of human LIME1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIME1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIME1
Synonyms LIME
Function Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
Cellular Location Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell
Tissue Location Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level)
Research Areas
Citations (0)

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LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).


Ahn, E., et al. Blood 107(4):1521-1527(2006)
Tedoldi, S., et al. Blood 107(1):213-221(2006)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Brdickova, N., et al. J. Exp. Med. 198(10):1453-1462(2003)
Hur, E.M., et al. J. Exp. Med. 198(10):1463-1473(2003)

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$ 295.00
$ 99.00
Cat# AP14247b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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