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M Mlkl Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - M Mlkl Antibody (C-term) AP14272b
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse testis lysates Lane 2: mouse liver lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Anti-Mlkl Antibody (C-term)at 1:1000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Anti-Mlkl Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    All lanes : Anti-Mlkl Antibody (C-term) at 1:1000 dilution Lane 1: mouse lung lysates Lane 2: mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: mouse liver lysates Lane 4: mouse testis whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Western blot analysis of lysate from mouse spleen tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - M Mlkl Antibody (C-term) AP14272b
    Mouse Mlkl Antibody (C-term) (Cat. #AP14272b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mlkl antibody detected the Mlkl protein (arrow).
  • SPECIFICATION
  • CITATIONS: 5
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9D2Y4
Other Accession NP_083281.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54317 Da
Antigen Region 444-472 aa
Additional Information
Gene ID 74568
Other Names Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}
Target/Specificity This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mlkl {ECO:0000312|EMBL:AAH23755.1, ECO:0000312|MGI:MGI:1921818}
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Cellular Location Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Tissue Location Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain
Research Areas
Citations ( 0 )

Background

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

References

Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

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$ 295.00
$ 99.00
Cat# AP14272b
Size:
Quantity:
(40 western blots)
Availability: In stock
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