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OTU7B Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - OTU7B Antibody (C-term) AP14281b
    OTU7B Antibody (C-term) (Cat. #AP14281b) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OTU7B antibody detected the OTU7B protein (arrow).
  • IHC-P - OTU7B Antibody (C-term) AP14281b
    OTU7B Antibody (C-term) (AP14281b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTU7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6GQQ9
Other Accession B2RUR8, NP_064590.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 92526 Da
Antigen Region 757-784 aa
Additional Information
Gene ID 56957
Other Names OTU domain-containing protein 7B, Cellular zinc finger anti-NF-kappa-B protein, Zinc finger A20 domain-containing protein 1, Zinc finger protein Cezanne, OTUD7B, ZA20D1
Target/Specificity This OTU7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-784 amino acids from the C-terminal region of human OTU7B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTU7B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ZA20D1
Function Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF- kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' or 'Lys-63'- linked polyubiquitin chains. In vitro, has preference for 'Lys- 11'-linked polyubiquitin chains; however such data are unsure in vivo. Hydrolyzes both linear and branched forms of polyubiquitin.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner
Tissue Location Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells
Research Areas
Citations (0)

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OTU7B has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.


Bremm, A., et al. Nat. Struct. Mol. Biol. 17(8):939-947(2010)
Jin, Z., et al. Cell 137(4):721-735(2009)
Enesa, K., et al. J. Biol. Chem. 283(27):18582-18590(2008)
Bohgaki, M., et al. Biochim. Biophys. Acta 1783(5):826-837(2008)
Enesa, K., et al. J. Biol. Chem. 283(11):7036-7045(2008)

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$ 295.00
$ 99.00
Cat# AP14281b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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