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Mouse Trib3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8K4K2 |
| Other Accession | NP_780302.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 39023 Da |
| Antigen Region | 306-334 aa |
| Gene ID | 228775 |
|---|---|
| Other Names | Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, Trib3, Nipk, Trb3 |
| Target/Specificity | This Mouse Trib3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the C-terminal region of mouse Trib3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Trib3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Trib3 |
|---|---|
| Synonyms | Nipk, Trb3 |
| Function | Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:17369260). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (By similarity). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (PubMed:12749859, PubMed:17369260). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (PubMed:12791994). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (PubMed:12791994). Interacts with the NF- kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases (By similarity). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230). |
| Cellular Location | Nucleus |
| Tissue Location | Highly expressed in liver. Not detected in heart, brain, spleen, lung, skeletal muscle, kidney or testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
Trib3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
References
Morse, E., et al. Am. J. Physiol. Renal Physiol. 299 (5), F965-F972 (2010) :
Dedhia, P.H., et al. Blood 116(8):1321-1328(2010)
Liew, C.W., et al. J. Clin. Invest. 120(8):2876-2888(2010)
Humphrey, R.K., et al. J. Biol. Chem. 285(29):22426-22436(2010)
Liu, J., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E565-E576 (2010) :
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