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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Trib3 Antibody (C-term)   

Mouse Trib3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Trib3 Antibody (C-term) AP14299b
    Mouse Trib3 Antibody (C-term) (Cat. #AP14299b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8K4K2
Other Accession NP_780302.2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39023 Da
Antigen Region 306-334 aa
Additional Information
Gene ID 228775
Other Names Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, Trib3, Nipk, Trb3
Target/Specificity This Mouse Trib3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the C-terminal region of mouse Trib3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Trib3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Trib3
Synonyms Nipk, Trb3
Function Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). Can inhibit APOBEC3A editing of nuclear DNA.
Cellular Location Nucleus
Tissue Location Highly expressed in liver. Not detected in heart, brain, spleen, lung, skeletal muscle, kidney or testis
Research Areas
Citations (0)

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Background

Trib3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.

References

Morse, E., et al. Am. J. Physiol. Renal Physiol. 299 (5), F965-F972 (2010) :
Dedhia, P.H., et al. Blood 116(8):1321-1328(2010)
Liew, C.W., et al. J. Clin. Invest. 120(8):2876-2888(2010)
Humphrey, R.K., et al. J. Biol. Chem. 285(29):22426-22436(2010)
Liu, J., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E565-E576 (2010) :

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Cat# AP14299b
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(40 western blots)
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