|Application ||WB, IHC-P, E|
|Calculated MW||68678 Da|
|Antigen Region||56-85 aa|
|Other Names||Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP|
|Target/Specificity||This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human AFP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AFP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.|
|Tissue Location||Plasma. Synthesized by the fetal liver and yolk sac|
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Provided below are standard protocols that you may find useful for product applications.
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. This protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Yamashita,T., Cancer Res. 68 (5), 1451-1461 (2008)
Chen,G.G., Eur J Surg Oncol 33 (7), 882-886 (2007)
Cajaiba,M.M., Pediatr. Dev. Pathol. 10 (3), 233-238 (2007)
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