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AFP Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1430c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
AFP Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P02771 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68678 Da |
AFP Antibody (Center) - Additional info | |
| Gene ID 174 | |
| Other Names AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin | |
| Target/Specificity This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the Center region of human AFP. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AFP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AFP Antibody (Center) - Protein Information | |
| Name AFP | |
| Synonyms HPAFP | |
| Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties | |
| Cellular Location Secreted. | |
| Tissue Location Plasma. Synthesized by the fetal liver and yolk sac | |
AFP Antibody (Center) - Related products
AM1835a: AFP Antibody (N-term)
AM1835b: AFP Antibody (Ascites)
AP1430a: AFP Antibody (N-term)
AP1430c: AFP Antibody (Center)
BP1430a: AFP Antibody (N-term) Blocking Peptide
BP1430c: AFP Antibody (Center) Blocking Peptide
AT1066a: AFP Antibody (monoclonal) (M01)
AJ1019a: AFP Antibody (C-term)
AFP Antibody (Center) - Application data
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Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) (Cat.#AP1430c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) (Cat.#AP1430c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AFP Antibody (Center) - Research Areas
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BACKGROUND
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
REFERENCES
Yamashita,T., Cancer Res. 68 (5), 1451-1461 (2008) Chen,G.G., Eur J Surg Oncol 33 (7), 882-886 (2007) Cajaiba,M.M., Pediatr. Dev. Pathol. 10 (3), 233-238 (2007)
