|Application ||WB, IHC-P, E|
|Other Accession||O08839, O08539, NP_647595.1, NP_647594.1, NP_004296.1|
|Calculated MW||64699 Da|
|Antigen Region||360-389 aa|
|Other Names||Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN1, AMPHL|
|Target/Specificity||This BIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids of human BIN1(O00499-1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BIN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.|
|Cellular Location||Isoform BIN1: Nucleus.|
|Tissue Location||Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier Isoform BIN1 is widely expressed with highest expression in skeletal muscle.|
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This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
Biffi, A., et al. Arch. Neurol. 67(6):677-685(2010) Seshadri, S., et al. JAMA 303(18):1832-1840(2010) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) Claeys, K.G., et al. Neurology 74(6):519-521(2010) Hong, T.T., et al. PLoS Biol. 8 (2), E1000312 (2010) :
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