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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Anti-Wnt-Pathway (plus Lgr5) Antibodies   >   M Cadherin (CDH15) Antibody (C-term)   

M Cadherin (CDH15) Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - M Cadherin (CDH15) Antibody (C-term) AP1435b
    Anti-CDH15 Antibody (C-term) at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - M Cadherin (CDH15) Antibody (C-term) AP1435b
    Western blot analysis of CDH15 (arrow) using rabbit polyclonal CDH15 Antibody (C-term) (Cat.#AP1435b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - M Cadherin (CDH15) Antibody (C-term) AP1435b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody (C-term) (Cat.#AP1435b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P55291
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 88916 Da
Antigen Region 739-767 aa
Additional Information
Gene ID 1013
Other Names Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3
Target/Specificity This M Cadherin (CDH15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 739-767 amino acids from the C-terminal region of human M Cadherin (CDH15).
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Cadherin (CDH15) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH15
Synonyms CDH14, CDH3
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in the brain and cerebellum.
Research Areas
Citations (0)
citation

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Background

CDH15 is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. This protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.

References

Kang,J.S., Proc. Natl. Acad. Sci. U.S.A. 100 (7), 3989-3994 (2003) Hollnagel,A., Mol. Cell. Biol. 22 (13), 4760-4770 (2002) Meigs,T.E., Proc. Natl. Acad. Sci. U.S.A. 98 (2), 519-524 (2001)

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$ 365.00
$ 140.00
Cat# AP1435b
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