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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF40 Antibody (C-term)   

RNF40 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RNF40 Antibody (C-term) AP14362B
    Western blot analysis of RNF40 Antibody (C-term) Pab (Cat. #AP14362b) pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. RNF40 Antibody (C-term) (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75150
Other Accession Q8CJB9, Q3U319, NP_055586.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 113650 Da
Antigen Region 697-725 aa
Additional Information
Gene ID 9810
Other Names E3 ubiquitin-protein ligase BRE1B, BRE1-B, 632-, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661
Target/Specificity This RNF40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697-725 amino acids from the C-terminal region of human RNF40.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF40 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF40
Synonyms BRE1B, KIAA0661
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
Cellular Location Nucleus.
Tissue Location Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine
Research Areas
Citations (0)

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The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.


Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010)
Kim, J., et al. Cell 137(3):459-471(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Zhu, B., et al. Mol. Cell 20(4):601-611(2005)

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$ 295.00
$ 99.00
Cat# AP14362B
(40 western blots)
Availability: In Stock
Bulk Size
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