|Application ||WB, IHC-P, E|
|Other Accession||O55203, Q9W676, NP_001124306.1, NP_001281.1|
|Calculated MW||42793 Da|
|Antigen Region||314-342 aa|
|Other Names||LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, LIM domain-binding factor CLIM1, LDB2, CLIM1|
|Target/Specificity||This LDB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the C-terminal region of human LDB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LDB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to the LIM domain of a wide variety of LIM domain- containing transcription factors.|
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Provided below are standard protocols that you may find useful for product applications.
Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hagg, S., et al. PLoS Genet. 5 (12), E1000754 (2009) :
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Kotaka, M., et al. J. Cell. Biochem. 78(4):558-565(2000)
Bach, I., et al. Nat. Genet. 22(4):394-399(1999)
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