|Application ||WB, E|
|Other Accession||Q9JJZ4, NP_057105.2|
|Calculated MW||35199 Da|
|Antigen Region||1-30 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 J1, Non-canonical ubiquitin-conjugating enzyme 1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e, UBE2J1, NCUBE1|
|Target/Specificity||This UBE2J1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2J1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2J1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type IV membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Oh, R.S., et al. J. Biol. Chem. 281(30):21480-21490(2006)
Wu, C.J., et al. EMBO J. 24(10):1886-1898(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
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