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UBE2J1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9Y385 |
| Other Accession | Q9JJZ4, NP_057105.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35199 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 51465 |
|---|---|
| Other Names | Ubiquitin-conjugating enzyme E2 J1, Non-canonical ubiquitin-conjugating enzyme 1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e, UBE2J1, NCUBE1 |
| Target/Specificity | This UBE2J1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2J1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UBE2J1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBE2J1 (HGNC:17598) |
|---|---|
| Synonyms | NCUBE1 |
| Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress (PubMed:28321712). Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis (PubMed:24020373). Acts also as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26 (PubMed:33472082). Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133 (PubMed:35831855). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type IV membrane protein |
| Tissue Location | Expressed in testes.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
References
Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Oh, R.S., et al. J. Biol. Chem. 281(30):21480-21490(2006)
Wu, C.J., et al. EMBO J. 24(10):1886-1898(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
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