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FOXD1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - FOXD1 Antibody (N-term) AP14388A
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP14388A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • WB - FOXD1 Antibody (N-term) AP14388A
    Anti-FOXD1 Antibody (N-term)at 1:2000 dilution + mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - FOXD1 Antibody (N-term) AP14388A
    Fluorescent image of B-3 cell stained with FOXD1 Antibody (N-term)(Cat#AP14388a/SA110804BE).B-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).FOXD1 immunoreactivity is localized to Nucleus significantly.
  • WB - FOXD1 Antibody (N-term) AP14388A
    FOXD1 Antibody (N-term) (Cat. #AP14388a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXD1 antibody detected the FOXD1 protein (arrow).
  • IHC-P - FOXD1 Antibody (N-term) AP14388A
    FOXD1 Antibody (N-term) (AP14388a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16676
Other Accession Q61345, NP_004463.1
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46140 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 2297
Other Names Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4, FREAC-4, FOXD1, FKHL8, FREAC4
Target/Specificity This FOXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXD1
Synonyms FKHL8, FREAC4
Function Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly (By similarity).
Cellular Location Nucleus.
Citations (0)

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This intronless gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in tumor formation.


Berg, D.T., et al. J. Biol. Chem. 280(15):14943-14947(2005)
Ernstsson, S., et al. J. Biol. Chem. 271(35):21094-21099(1996)
Larsson, C., et al. Genomics 30(3):464-469(1995)
Pierrou, S., et al. EMBO J. 13(20):5002-5012(1994)

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$ 295.00
$ 99.00
Cat# AP14388A
(40 western blots)
Availability: In Stock
Bulk Size
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