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DPPA4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IHC-P - DPPA4 Antibody (N-term) AP1438a
    AP1438a staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - DPPA4 Antibody (N-term) AP1438a
    Anti-DPPA4 Antibody (N-term) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DPPA4 Antibody (N-term) AP1438a
    Western blot analysis of DPPA4 Antibody (N-term) (Cat. #AP1438a) in CEM cell line lysates (35ug/lane). DPPA4 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7L190
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33541 Da
Antigen Region 50-79 aa
Additional Information
Gene ID 55211
Other Names Developmental pluripotency-associated protein 4, DPPA4
Target/Specificity This DPPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human DPPA4.
Dilution IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDPPA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DPPA4
Function May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
Cellular Location Nucleus.
Research Areas
Citations (0)

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DppA4 is a member of the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog. It may play a role in maintaining cell pluripotentiality.


Bortvin,A., Development 130 (8), 1673-1680 (2003

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$ 295.00
$ 99.00
Cat# AP1438a
(40 western blots)
Availability: In Stock
Bulk Size
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