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PPEF1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPEF1 Antibody (Center) AP14395c
    Western blot analysis of PPEF1 (arrow) using rabbit polyclonal PPEF1 Antibody (Center) (Cat. #AP14395c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPEF1 gene.
    detail
  • WB - PPEF1 Antibody (Center) AP14395c
    PPEF1 Antibody (Center) (Cat. #AP14395c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PPEF1 antibody detected the PPEF1 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O14829
Other Accession NP_006231.2, NP_689410.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 75792 Da
Antigen Region 298-327 aa
Additional Information
Gene ID 5475
Other Names Serine/threonine-protein phosphatase with EF-hands 1, PPEF-1, Protein phosphatase with EF calcium-binding domain, PPEF, Serine/threonine-protein phosphatase 7, PP7, PPEF1, PPEF, PPP7C
Target/Specificity This PPEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human PPEF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPEF1
Synonyms PPEF, PPP7C
Function May have a role in the recovery or adaptation response of photoreceptors. May have a role in development.
Tissue Location Detected in retina and retinal derived Y-79 retinoblastoma cells. Also found in fetal brain
Research Areas
Citations (0)
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Background

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.

References

Ross, M.T., et al. Nature 434(7031):325-337(2005)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
Kutuzov, M.A., et al. Biochem. Biophys. Res. Commun. 293(3):1047-1052(2002)
Ramulu, P., et al. Mol. Cell. Biol. 21(24):8605-8614(2001)

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$ 182.50
$ 70.00
Cat# AP14395c
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