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RNF144A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RNF144A Antibody (Center) AP14399c
    RNF144A Antibody (Center) (Cat. #AP14399c) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNF144A antibody detected the RNF144A protein (arrow).
  • IHC-P - RNF144A Antibody (Center) AP14399c
    RNF144A Antibody (Center) (AP14399c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF144A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50876
Other Accession NP_055561.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32890 Da
Antigen Region 156-184 aa
Additional Information
Gene ID 9781
Other Names E3 ubiquitin-protein ligase RNF144A, 632-, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4, RNF144A, KIAA0161, RNF144, UBCE7IP4
Target/Specificity This RNF144A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human RNF144A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF144A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF144A
Synonyms KIAA0161, RNF144, UBCE7IP4
Function E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
Cellular Location Cell membrane; Single-pass membrane protein. Cytoplasmic vesicle membrane
Research Areas
Citations (0)

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The protein encoded by this protein contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. The mouse counterpart of this protein has been shown to interact with Ube2l3/UbcM4, which is an ubiquitin-conjugating enzyme involved in embryonic development.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Adkins, D.E., et al. Mol. Psychiatry (2010) In press :
Hoja, M.R., et al. Exp. Cell Res. 259(1):239-246(2000)
Martinez-Noel, G., et al. FEBS Lett. 454(3):257-261(1999)

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Cat# AP14399c
(40 western blots)
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