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PPEF2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPEF2 Antibody (Center) AP14402c
    Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody (Center)(Cat. #AP14402c). AP14402c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
    detail
  • IHC-P - PPEF2 Antibody (Center) AP14402c
    PPEF2 Antibody (Center) (AP14402c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPEF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O14830
Other Accession NP_006230.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86518 Da
Antigen Region 304-333 aa
Additional Information
Gene ID 5470
Other Names Serine/threonine-protein phosphatase with EF-hands 2, PPEF-2, PPEF2
Target/Specificity This PPEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human PPEF2.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPEF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPEF2
Function May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission.
Cellular Location Cytoplasm. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment Note=Localized to photoreceptors, PPEF-2(L) is at least 2 fold more abundant in rod inner segments than in the outer segments
Tissue Location Retinal specific.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. [provided by RefSeq].

References

Kutuzov, M.A., et al. Biochem. Biophys. Res. Commun. 293(3):1047-1052(2002)
Ramulu, P., et al. Mol. Cell. Biol. 21(24):8605-8614(2001)
Sherman, P.M., et al. Proc. Natl. Acad. Sci. U.S.A. 94(21):11639-11644(1997)

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$ 365.00
$ 140.00
Cat# AP14402c
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