|Application ||WB, FC, E|
|Other Accession||O96013, NP_081746.1|
|Calculated MW||64623 Da|
|Antigen Region||187-216 aa|
|Other Names||Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, Pak4, Kiaa1142|
|Target/Specificity||This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of mouse Pak4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Pak4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.|
|Cellular Location||Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity).
Liu, Y., et al. Oncogene 29(44):5883-5894(2010)
Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009) :
Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008)
Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008)
Danzer, K.M., et al. J. Neurochem. 103(6):2401-2407(2007)
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