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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Pak4 Antibody (Center)   

Mouse Pak4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Pak4 Antibody (Center) AP14453C
    Mouse Pak4 Antibody (Center) (Cat. #AP14453c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).
  • WB - Mouse Pak4 Antibody (Center) AP14453C
    Mouse Pak4 Antibody (Center) (Cat. #AP14453c) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).
  • FC - Mouse Pak4 Antibody (Center) AP14453C
    Mouse Pak4 Antibody (Center) (Cat. #AP14453c) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q8BTW9
Other Accession O96013, NP_081746.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64623 Da
Antigen Region 187-216 aa
Additional Information
Gene ID 70584
Other Names Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, Pak4, Kiaa1142
Target/Specificity This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of mouse Pak4.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Pak4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pak4
Synonyms Kiaa1142
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O96013}. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways. {ECO:0000250|UniProtKB:O96013}
Research Areas
Citations (0)

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Background

Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity).

References

Liu, Y., et al. Oncogene 29(44):5883-5894(2010)
Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009) :
Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008)
Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008)
Danzer, K.M., et al. J. Neurochem. 103(6):2401-2407(2007)

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$ 295.00
$ 99.00
Cat# AP14453C
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Quantity:
(40 western blots)
Availability: In Stock
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