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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Wee2 Antibody (Center)   

Mouse Wee2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Wee2 Antibody (Center) AP14472c
    Mouse Wee2 Antibody (Center) (Cat. #AP14472c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Wee2 antibody detected the Wee2 protein (arrow).
  • IHC-P - Mouse Wee2 Antibody (Center) AP14472c
    Mouse Wee2 Antibody (Center) (AP14472c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Wee2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q66JT0
Other Accession NP_958758.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62343 Da
Antigen Region 364-393 aa
Additional Information
Gene ID 381759
Other Names Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase, mWee1B, Wee2, Wee1b
Target/Specificity This Mouse Wee2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the Central region of mouse Wee2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Wee2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Wee2
Synonyms Wee1b
Function Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
Cellular Location Cytoplasm. Nucleus. Note=Localizes mainly in the nucleus. Exported from the nucleus to the cytoplasm before germinal vesicle breakdown (GVBD), allowing meiosis resumption
Tissue Location Ovary-specific..
Research Areas
Citations (0)

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Phosphorylates and inhibits CDK1. May act as a negative regulator of entry into mitosis (G2 to M transition).


Oh, J.S., et al. J. Cell Biol. 188(2):199-207(2010)
Joshi, S., et al. BMC Dev. Biol. 7, 67 (2007) :
Han, S.J., et al. Curr. Biol. 15(18):1670-1676(2005)
Nakanishi, M., et al. Genes Cells 5(10):839-847(2000)

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Cat# AP14472c
(40 western blots)
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