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CDCA8 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDCA8 Antibody (Center) AP1447c
    Anti-CDCA8 Antibody (Center) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q53HL2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31323 Da
Antigen Region 115-144 aa
Additional Information
Gene ID 55143
Other Names Borealin, Cell division cycle-associated protein 8, Dasra-B, hDasra-B, Pluripotent embryonic stem cell-related gene 3 protein, CDCA8, PESCRG3
Target/Specificity This CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the Central region of human CDCA8.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDCA8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDCA8
Synonyms PESCRG3
Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Cellular Location Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis Colocalizes with SENP3 in the nucleolus in interphase cells
Research Areas
Citations ( 0 )

Background

CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.

References

Sampath, S.C., et al., Cell 118(2):187-202 (2004).
Gassmann, R., et al., J. Cell Biol. 166(2):179-191 (2004).

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$ 182.50
$ 70.00
Cat# AP1447c
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