|Application ||WB, IHC-P, E|
|Calculated MW||29489 Da|
|Antigen Region||157-186 aa|
|Other Names||Chymotrypsin-like elastase family member 3A, Elastase IIIA, Elastase-3A, Protease E, CELA3A, ELA3, ELA3A|
|Target/Specificity||This CELA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human CELA3A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CELA3A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Efficient protease with alanine specificity but only little elastolytic activity.|
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Provided below are standard protocols that you may find useful for product applications.
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.
Shimada, S., et al. Int. J. Mol. Med. 10(5):599-603(2002)
Shirasu, Y., et al. J. Biochem. 104(2):259-264(1988)
Tani, T., et al. J. Biol. Chem. 263(3):1231-1239(1988)
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