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NHEJ1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NHEJ1 Antibody (C-term) AP14503b
    NHEJ1 Antibody (C-term) (Cat. #AP14503b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NHEJ1 antibody detected the NHEJ1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H9Q4
Other Accession NP_079058.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33337 Da
Antigen Region 268-296 aa
Additional Information
Gene ID 79840
Other Names Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF
Target/Specificity This NHEJ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-296 amino acids from the C-terminal region of human NHEJ1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNHEJ1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NHEJ1
Synonyms XLF
Function DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Cellular Location Nucleus
Tissue Location Ubiquitously expressed.
Citations (0)

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Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.


Malivert, L., et al. J. Biol. Chem. 285(34):26475-26483(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Andres, S.N., et al. Mol. Cell 28(6):1093-1101(2007)
Tsai, C.J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(19):7851-7856(2007)

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$ 295.00
$ 99.00
Cat# AP14503b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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