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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ASAP1 Antibody (Center)   

ASAP1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ASAP1 Antibody (Center) AP14505C
    ASAP1 Antibody (Center) (Cat. #AP14505c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the ASAP1 antibody detected the ASAP1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9ULH1
Other Accession Q1AAU6, Q9QWY8, O97902, NP_060952.2
Reactivity Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 125498 Da
Antigen Region 360-388 aa
Additional Information
Gene ID 50807
Other Names Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4, 5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP, ASAP1, DDEF1, KIAA1249
Target/Specificity This ASAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the Central region of human ASAP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASAP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASAP1
Synonyms DDEF1, KIAA1249
Function Possesses phosphatidylinositol 4,5-bisphosphate- dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
Cellular Location Cytoplasm. Membrane. Note=Predominantly cytoplasmic. Partially membrane- associated.
Research Areas
Citations (0)

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Background

Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).

References

Muller, T., et al. Oncogene 29(16):2393-2403(2010)
Nat. Genet. 41(7):824-828(2009)
Jian, X., et al. J. Biol. Chem. 284(3):1652-1663(2009)
Inoue, H., et al. Mol. Biol. Cell 19(10):4224-4237(2008)
Lin, D., et al. Cancer Res. 68(11):4352-4359(2008)

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$ 295.00
$ 99.00
Cat# AP14505C
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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