|Application ||WB, E|
|Other Accession||NP_001135765.1, NP_055385.3|
|Calculated MW||56928 Da|
|Antigen Region||445-473 aa|
|Other Names||ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP3, ARFGAP1|
|Target/Specificity||This ARFGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human ARFGAP3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARFGAP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment|
|Tissue Location||Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Saitoh, A., et al. J. Biol. Chem. 284(20):13948-13957(2009)
Weimer, C., et al. J. Cell Biol. 183(4):725-735(2008)
Frigerio, G., et al. Traffic 8(11):1644-1655(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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