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PTPRN Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PTPRN Antibody (Center) AP14523c
    PTPRN Antibody (Center) (Cat. #AP14523c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRN antibody detected the PTPRN protein (arrow).
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  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16849
Other Accession NP_002837.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 105848 Da
Antigen Region 288-316 aa
Additional Information
Gene ID 5798
Other Names Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2, PTPRN, ICA3, ICA512
Target/Specificity This PTPRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-316 amino acids from the Central region of human PTPRN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTPRN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPRN
Synonyms ICA3, ICA512
Function Plays a role in vesicle-mediated secretory processes (PubMed:24843546). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets (By similarity). Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed:24843546). Plays a role in insulin secretion in response to glucose stimuli (PubMed:24843546). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain (By similarity). In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle- stimulating hormone (FSH) (By similarity). Required to maintain normal levels of renin expression and renin release (By similarity). Seems to lack intrinsic enzyme activity (By similarity). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity).
Cellular Location Membrane {ECO:0000250|UniProtKB:Q63259}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q63259}. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type I membrane protein. Perikaryon {ECO:0000250|UniProtKB:Q63259}. Cell projection, axon {ECO:0000250|UniProtKB:Q63259}. Cell junction, synapse {ECO:0000250|UniProtKB:Q63259}. Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q63259}. Endosome {ECO:0000250|UniProtKB:Q63259}. Note=Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules (PubMed:25561468). Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes {ECO:0000250|UniProtKB:Q63259, ECO:0000269|PubMed:25561468} ICA512-cleaved cytosolic fragment: Nucleus
Tissue Location Expression is restricted to neuroendocrine cells. Found in pancreas, brain and pituitary
Research Areas
Citations ( 0 )

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus.

References

Yu, L., et al. J. Immunol. Methods 353 (1-2), 20-23 (2010) :
Honeyman, M.C., et al. J. Immunol. 184(4):2204-2210(2010)
Weenink, S.M., et al. J. Autoimmun. 33(2):147-154(2009)
Burbelo, P.D., et al. Diabetes Care 31(9):1824-1826(2008)
Williams, A.J., et al. Diabetologia 51(8):1444-1448(2008)

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$ 295.00
$ 99.00
Cat# AP14523c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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