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>   home   >   Products   >   Primary Antibodies   >   POLI Antibody (C-term)   

POLI Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLI Antibody (C-term) AP14526b
    POLI Antibody (C-term) (Cat. #AP14526b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POLI antibody detected the POLI protein (arrow).
  • IHC-P - POLI Antibody (C-term) AP14526b
    POLI Antibody (C-term) (AP14526b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLI Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UNA4
Other Accession NP_009126.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83006 Da
Antigen Region 673-701 aa
Additional Information
Gene ID 11201
Other Names DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B
Target/Specificity This POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673-701 amino acids from the C-terminal region of human POLI.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLI
Synonyms RAD30B
Function Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Cellular Location Nucleus. Note=Accumulates at replication forks after DNA damage
Tissue Location Ubiquitous. Highly expressed in testis.
Citations (0)

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Background

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

References

Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Kazakov, A.A., et al. Biochemistry Mosc. 75(7):905-911(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :

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$ 295.00
$ 99.00
Cat# AP14526b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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