|Application ||WB, IHC-P, E|
|Calculated MW||37158 Da|
|Antigen Region||288-317 aa|
|Other Names||Laforin, 313-, Glucan phosphatase, Lafora PTPase, LAFPTPase, EPM2A|
|Target/Specificity||This EPM2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human EPM2A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPM2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has both dual-specificity protein phosphatase and glucan phosphatase activities. Together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1.|
|Cellular Location||Cytoplasm Note=Under glycogenolytic conditions localizes to the nucleus Isoform 2: Endoplasmic reticulum. Cell membrane. Nucleus. Note=Also found in the nucleus Isoform 5: Cytoplasm. Nucleus.|
|Tissue Location||Expressed in heart, skeletal muscle, kidney, pancreas and brain. Isoform 4 is also expressed in the placenta|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
EPM2A is a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations have been associated with myoclonic epilepsy of Lafora.
Minassian B.A., Nat. Genet. 20:171-174(1998).
Ganesh S., Hum. Mol. Genet. 9:2251-2261(2000).
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