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EIF2AK3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF2AK3 Antibody (N-term) AP14551A
    EIF2AK3 Antibody (N-term) (Cat. #AP14551a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EIF2AK3 antibody detected the EIF2AK3 protein (arrow).
  • IHC-P - EIF2AK3 Antibody (N-term) AP14551A
    EIF2AK3 Antibody (N-term) (AP14551a)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2AK3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NZJ5
Other Accession NP_004827.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 125216 Da
Antigen Region 73-102 aa
Additional Information
Gene ID 9451
Other Names Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK
Target/Specificity This EIF2AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human EIF2AK3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF2AK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2AK3
Synonyms PEK, PERK
Function Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)- induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Ubiquitous. A high level expression is seen in secretory tissues
Research Areas
Citations (0)

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Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

References

Xu, H., et al. Toxicology 277 (1-3), 1-5 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kim, K.W., et al. Oncogene 29(22):3241-3251(2010)
Lee do, Y., et al. PLoS ONE 5 (5), E10489 (2010) :

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Cat# AP14551A
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(40 western blots)
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