- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||Q5U300, Q29504, Q02053, A3KMV5, NP_695012.1, NP_003325.2|
|Predicted||Bovine, Mouse, Rabbit, Rat|
|Calculated MW||117849 Da|
|Antigen Region||1-30 aa|
|Other Names||Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1|
|Target/Specificity||This UBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus Isoform 2: Cytoplasm|
|Tissue Location||Detected in erythrocytes (at protein level). Ubiquitous.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Burkhardt, J., et al. J. Rheumatol. 36(10):2149-2157(2009)
Su, Z.L., et al. Leuk. Lymphoma 49(9):1821-1822(2008)
Ramser, J., et al. Am. J. Hum. Genet. 82(1):188-193(2008)
Carbia-Nagashima, A., et al. Cell 131(2):309-323(2007)
Jin, J., et al. Nature 447(7148):1135-1138(2007)
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