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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   UBA1 Antibody (N-term)   

UBA1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBA1 Antibody (N-term) AP14555a
    UBA1 Antibody (N-term) (Cat. #AP14555a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBA1 antibody detected the UBA1 protein (arrow).
  • IHC-P - UBA1 Antibody (N-term) AP14555a
    UBA1 Antibody (N-term) (AP14555a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P22314
Other Accession Q5U300, Q29504, Q02053, A3KMV5, NP_695012.1, NP_003325.2
Reactivity Human
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 117849 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 7317
Other Names Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1
Target/Specificity This UBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBA1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA1
Synonyms A1S9T, UBE1
Function Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
Cellular Location Cytoplasm. Mitochondrion. Nucleus Isoform 2: Cytoplasm
Tissue Location Detected in erythrocytes (at protein level). Ubiquitous.
Research Areas
Citations ( 0 )

Background

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

References

Burkhardt, J., et al. J. Rheumatol. 36(10):2149-2157(2009)
Su, Z.L., et al. Leuk. Lymphoma 49(9):1821-1822(2008)
Ramser, J., et al. Am. J. Hum. Genet. 82(1):188-193(2008)
Carbia-Nagashima, A., et al. Cell 131(2):309-323(2007)
Jin, J., et al. Nature 447(7148):1135-1138(2007)

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$ 295.00
$ 99.00
Cat# AP14555a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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