|Application ||WB, E|
|Other Accession||Q5M939, Q8BY71, NP_003633.1|
|Calculated MW||49513 Da|
|Antigen Region||21-50 aa|
|Other Names||Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1|
|Target/Specificity||This HAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human HAT1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HAT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.|
|Cellular Location||Isoform A: Nucleus matrix.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
Saade, E., et al. Proteomics 9(21):4934-4943(2009)
Miyamoto, N., et al. J. Biol. Chem. 283(26):18218-18226(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Benson, L.J., et al. J. Biol. Chem. 282(2):836-842(2007)
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