SIGLEC8 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q9NYZ4 |
Other Accession | NP_055257.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 54042 Da |
Antigen Region | 94-122 aa |
Gene ID | 27181 |
---|---|
Other Names | Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2, SIGLEC8, SAF2 |
Target/Specificity | This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human SIGLEC8. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SIGLEC8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SIGLEC8 |
---|---|
Synonyms | SAF2 |
Function | Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, PubMed:10625619). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6- sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658). |
Cellular Location | Membrane; Single-pass type I membrane protein. |
Tissue Location | Expressed specifically on red blood cells namely basophil, mast cells and eosinophils. |
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Background
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).
References
Gao, P.S., et al. Eur. J. Hum. Genet. 18(6):713-719(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009)
Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009)
von Gunten, S., et al. Autoimmun Rev 7(6):453-456(2008)
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