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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SIGLEC8 Antibody (N-term)   

SIGLEC8 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SIGLEC8 Antibody (N-term) AP14564a
    SIGLEC8 Antibody (N-term) (Cat. #AP14564a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).
  • IHC-P - SIGLEC8 Antibody (N-term) AP14564a
    SIGLEC8 Antibody (N-term) (AP14564a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NYZ4
Other Accession NP_055257.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54042 Da
Antigen Region 94-122 aa
Additional Information
Gene ID 27181
Other Names Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2, SIGLEC8, SAF2
Target/Specificity This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human SIGLEC8.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIGLEC8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIGLEC8
Synonyms SAF2
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed specifically on eosinophils.
Research Areas
Citations (0)

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Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).

References

Gao, P.S., et al. Eur. J. Hum. Genet. 18(6):713-719(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009)
Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009)
von Gunten, S., et al. Autoimmun Rev 7(6):453-456(2008)

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Cat# AP14564a
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(40 western blots)
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