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SIRT5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SIRT5 Antibody (Center) AP14574C
    SIRT5 Antibody (Center) (Cat. #AP14574c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SIRT5 antibody detected the SIRT5 protein (arrow).
  • WB - SIRT5 Antibody (Center) AP14574C
    Western blot analysis of SIRT5 (arrow) using rabbit polyclonal SIRT5 Antibody (Center) (Cat. #AP14574c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SIRT5 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NXA8
Other Accession Q96S44, NP_036373.1, NP_112534.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33881 Da
Antigen Region 99-127 aa
Additional Information
Gene ID 23408
Other Names NAD-dependent protein deacylase sirtuin-5, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03160}, 351- {ECO:0000255|HAMAP-Rule:MF_03160}, Regulatory protein SIR2 homolog 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2-like protein 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2L5
Target/Specificity This SIRT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human SIRT5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}
Synonyms SIR2L5
Function NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.
Cellular Location Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus Isoform 2: Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03160, ECO:0000269|PubMed:21143562}
Tissue Location Widely expressed.
Research Areas
Citations (0)

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This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.


Schlicker, C., et al. J. Mol. Biol. 382(3):790-801(2008)
Yamamoto, H., et al. Mol. Endocrinol. 21(8):1745-1755(2007)
Chowdari, K.V., et al. Genes Brain Behav. 6(3):229-239(2007)
Mahlknecht, U., et al. Cytogenet. Genome Res. 112 (3-4), 208-212 (2006) :
Michishita, E., et al. Mol. Biol. Cell 16(10):4623-4635(2005)

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Cat# AP14574C
(40 western blots)
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