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EWSR1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - EWSR1 Antibody (C-term) AP14576b
    EWSR1 Antibody (C-term) (Cat. #AP14576b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow).
  • IF - EWSR1 Antibody (C-term) AP14576b
    Fluorescent confocal image of Hela cell stained with EWSR1 Antibody (C-term)(Cat#AP14576b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01844
Other Accession Q61545, NP_005234.1, NP_001156757.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68478 Da
Antigen Region 623-652 aa
Additional Information
Gene ID 2130
Other Names RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein, EWSR1, EWS
Target/Specificity This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 623-652 amino acids from the C-terminal region of human EWSR1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEWSR1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EWSR1
Synonyms EWS
Function Might normally function as a transcriptionnal repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq].


Lagirand-Cantaloube, J., et al. Biochem. Biophys. Res. Commun. 399(4):705-710(2010)
Kumagai, A., et al. Am. J. Clin. Pathol. 134(2):323-331(2010)
Aryee, D.N., et al. Cancer Res. 70(10):4015-4023(2010)
Riggi, N., et al. Genes Dev. 24(9):916-932(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP14576b
(40 western blots)
Availability: In Stock
Bulk Size
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