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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HSD3B1 Antibody (N-term)   

HSD3B1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HSD3B1 Antibody (N-term) AP14585a
    Anti-HSD3B1 Antibody (N-term) at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HSD3B1 Antibody (N-term) AP14585a
    HSD3B1 Antibody (N-term) (Cat. #AP14585a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).
  • WB - HSD3B1 Antibody (N-term) AP14585a
    Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term) (Cat. #AP14585a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P14060
Other Accession NP_000853.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42252 Da
Antigen Region 26-55 aa
Additional Information
Gene ID 3283
Other Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxy-5-ene steroid dehydrogenase, Progesterone reductase, Steroid Delta-isomerase, Delta-5-3-ketosteroid isomerase, HSD3B1, 3BH, HSDB3A
Target/Specificity This HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human HSD3B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSD3B1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSD3B1
Synonyms 3BH, HSDB3A
Function 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein
Tissue Location Placenta and skin. Predominantly expressed in mammary gland tissue
Research Areas
Citations (0)

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Background

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.

References

Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Shimodaira, M., et al. Eur. J. Endocrinol. 163(4):671-680(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Thomas, J.L., et al. J. Steroid Biochem. Mol. Biol. 120 (4-5), 192-199 (2010) :

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$ 295.00
$ 99.00
Cat# AP14585a
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Quantity:
(40 western blots)
Availability: In Stock
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