|Application ||WB, E|
|Other Accession||Q9Z2X3, Q9Z2X2, NP_002805.1, NP_736606.1|
|Calculated MW||24428 Da|
|Antigen Region||184-213 aa|
|Other Names||26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, p28(GANK), PSMD10|
|Target/Specificity||This PSMD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the C-terminal region of human PSMD10.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMD10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
Meng, Y., et al. Cancer Lett. 297(1):9-17(2010)
Man, J.H., et al. J. Clin. Invest. 120(8):2829-2841(2010)
Piton, A., et al. Mol. Psychiatry (2010) In press :
Serquera, D., et al. Biophys. J. 98(7):1294-1301(2010)
Tang, S., et al. Cancer Biol. Ther. 9(2):88-95(2010)
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