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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NEDD9 Antibody (Center)   

NEDD9 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NEDD9 Antibody (Center) AP14592c
    NEDD9 Antibody (Center) (Cat. #AP14592c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NEDD9 antibody detected the NEDD9 protein (arrow).
  • IHC-P - NEDD9 Antibody (Center) AP14592c
    NEDD9 Antibody (Center) (AP14592c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEDD9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q14511
Other Accession NP_006394.1, NP_892011.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 92861 Da
Antigen Region 335-363 aa
Additional Information
Gene ID 4739
Other Names Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, CAS-L, CasL, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, NEDD-9, Renal carcinoma antigen NY-REN-12, p105, Enhancer of filamentation 1 p55, NEDD9, CASL, CASS2
Target/Specificity This NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the Central region of human NEDD9.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEDD9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD9
Synonyms CASL, CASS2
Function Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Cellular Location Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle
Tissue Location Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues
Research Areas
Citations (0)

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Background

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.

References

Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Lucas, J.T. Jr., et al. Oncogene 29(31):4449-4459(2010)
Tedde, A., et al. Neurosci. Lett. 477(3):121-123(2010)
Malleter, M., et al. Int. J. Mol. Med. 25(6):897-903(2010)
Laumet, G., et al. J. Alzheimers Dis. 20(4):1181-1188(2010)

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$ 295.00
$ 99.00
Cat# AP14592c
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(40 western blots)
Availability: In Stock
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