|Application ||IF, WB, E|
|Other Accession||NP_001015892.1, NP_003178.1|
|Calculated MW||28974 Da|
|Antigen Region||1-30 aa|
|Other Names||Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G, STAF31/32, Transcription initiation factor TFIID 31 kDa subunit, TAFII-31, TAFII31, Transcription initiation factor TFIID 32 kDa subunit, TAFII-32, TAFII32, TAF9, TAF2G, TAFII31|
|Target/Specificity||This TAF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TAF9.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TAF9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.|
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Provided below are standard protocols that you may find useful for product applications.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants encoding different isoforms.
Kim, D.H., et al. BMB Rep 42(7):411-417(2009)
Sengupta, T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(11):4213-4218(2009)
Liu, X., et al. Mol. Cell. Biol. 28(1):108-121(2008)
McKeegan, K.S., et al. Mol. Cell. Biol. 27(19):6782-6793(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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