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WNT3A Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P56704 |
| Other Accession | P17553, P56703, P31285, P27467, Q2LMP1, NP_149122.1 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 39365 Da |
| Antigen Region | 151-180 aa |
| Gene ID | 89780 |
|---|---|
| Other Names | Protein Wnt-3a, WNT3A |
| Target/Specificity | This WNT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human WNT3A. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | WNT3A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WNT3A |
|---|---|
| Function | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720). |
| Cellular Location | Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:P27467}. Secreted |
| Tissue Location | Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
References
Sengupta, P.K., et al. J. Biol. Chem. 285(41):31164-31173(2010)
Hubner, R., et al. Biochem. Biophys. Res. Commun. 400(3):358-362(2010)
von Marschall, Z., et al. Biochem. Biophys. Res. Commun. 400(3):299-304(2010)
Shin, H., et al. J. Dermatol. Sci. 58(2):91-96(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
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