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E2F3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - E2F3 Antibody (Center) AP14598c
    Anti-E2F3 Antibody (Center) at 1:1000 dilution + mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - E2F3 Antibody (Center) AP14598c
    E2F3 Antibody (Center) (Cat. #AP14598c) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the E2F3 antibody detected the E2F3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00716
Other Accession O35261, NP_001940.1
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49162 Da
Antigen Region 151-180 aa
Additional Information
Gene ID 1871
Other Names Transcription factor E2F3, E2F-3, E2F3, KIAA0075
Target/Specificity This E2F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human E2F3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE2F3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F3
Synonyms KIAA0075
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).
Cellular Location Nucleus.
Citations (0)

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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.


Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
Biswas, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6976-6981(2010)
Martinez, L.A., et al. Mol. Cell. Biol. 30(2):524-536(2010)
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Madhavan, J., et al. Mol. Vis. 15, 235-240 (2009) :

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$ 295.00
$ 99.00
Cat# AP14598c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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